FastQCFastQC Report
Mon 27 Feb 2023
SRR3126395.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126395.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652339
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275921.0402893446124346No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29000.10933745648652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106750.086.732371
ATAGGGC16150.078.569853
ATAGCGG6150.071.944471
ACGGGAT6000.071.277894
TAGACGG2550.070.1436161
TAGGGCA18000.069.450254
GTAGGGC13350.068.998473
TAGAGGG47600.068.6497652
AGTAGGG40250.068.5698852
AGGGCAT24700.068.3065265
CGAAGGG27750.068.11212
CGTAGGG8400.067.727852
TATAGGG22150.066.864882
TAGCGGG16550.066.478172
AGTAAGG14950.066.433331
GTAGGGA17900.066.4250953
AGAGGGC39550.066.305933
ATAGAGG16450.066.098371
ATAGGGA22150.065.773763
CCGTACA10150.065.748673
Produced by FastQC (version 0.11.5)