FastQCFastQC Report
Mon 27 Feb 2023
SRR3126393.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126393.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3050236
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353461.1587955817189226No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32410.10625407345529986No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT130050.086.981111
ACGGGAT7200.077.677074
TAGACGG3350.077.221481
ATAGGGC20100.074.588693
CGTAGGG8000.074.021682
GTAGGGC15650.072.674063
TAGGGCA20700.072.4267044
ACGGGTA2800.072.175344
ATAGCGG6800.071.935741
ATAGGGT15000.069.5568853
AGTAGGG45750.068.9302442
CGAAGGG30200.068.4737552
ATAGGGA28350.068.3002853
AGGGATG41150.068.298285
AGGGCAT28400.067.849075
TAGAGGG53600.067.427952
TATAGGG26250.067.3188862
AGAGGGC50250.066.592043
TAAGGGA26400.066.402333
TAGCGGG18900.066.145192
Produced by FastQC (version 0.11.5)