FastQCFastQC Report
Mon 27 Feb 2023
SRR3126392.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126392.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109908
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167060.791788077963589No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31170.14773155985948203No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG30960.14673625579883104No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGTCAAAG27300.12938952788462815No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC26330.12479217103304979No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG23580.11175842738166783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58100.084.15091
AGTAGGG33750.072.29552
AGGGATG26750.072.031655
TAGGGCA10900.072.003434
GTAGGGT9850.071.567913
ACGGGAT4550.071.269054
ATAGGGC11750.070.7943653
GAATAGG13700.068.695581
CGAAGGG19850.068.683942
ACGGGTA2750.068.358194
ATAGGGA17950.068.072573
AATAGGG31950.067.539752
AGGGAAT23750.067.476735
GTAGGGA15550.067.396563
CGTAGGG6000.067.385252
AGTAAGG13900.067.030081
AGGGCAT20250.066.839125
AGGGTAT10900.066.829545
TAAGCGG4950.066.54451
AGGGCAC13950.066.367475
Produced by FastQC (version 0.11.5)