FastQCFastQC Report
Mon 27 Feb 2023
SRR3126391.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126391.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185642
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171270.7836141508993696No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG35100.16059354642709098No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30780.14082818686683365No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC28340.12966441896705866No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG26130.11955297345127885No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGTCAAAG25670.11744832868328847No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT22210.10161773977623051No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59600.083.1618651
ACGGGAT4350.074.540374
GTAGGGA13850.072.270583
AGGGCAT18850.071.299485
TAAGGGA18100.071.138333
AGTAGGG34150.071.005292
AGGGATG28700.070.4073945
TAGGGCA11100.069.854224
ATAGGGC12050.069.806793
CGTAGGG7350.069.690062
TAGGGAT12900.069.214264
GTACGGG4450.068.641232
AGTAAGG14450.068.3409961
GTAGGGT10200.068.185693
ATAGGGA19300.068.176143
GGTAAGG13150.067.945031
GAATAGG12950.067.5418551
TAGTAGG9750.067.040931
CGAAGGG21550.066.727652
AGGGAAT26050.066.5656745
Produced by FastQC (version 0.11.5)