FastQCFastQC Report
Mon 27 Feb 2023
SRR3126390.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126390.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424377
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215060.8870732563458571No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG36850.15199781222144906No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35750.14746056409543565No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC33350.13756111363867912No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG31700.13075524144965903No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTT24950.10291303704003131No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75000.082.8954851
CGTAGGG8200.073.361812
AGGGATG28900.072.528875
AGTAGGG40400.071.310432
ATAGCGG3950.069.0544361
TAGGGCG4700.068.99624
ATAAGGG31150.068.798782
ATAGGGA21850.068.614073
AATAGGG34400.068.583432
ACGGGTC3500.068.481954
TATAGGG17950.067.812422
ATAGGGC13600.067.731563
TAAGGGA21400.067.421523
AGTAAGG17300.067.4165041
TAGAGGG42600.067.407082
TAGGGCA13400.067.339584
AGGGCAT21550.067.170325
GTAGGGT9850.066.798353
TAGGGAC11100.066.4738164
ACGGGAT5450.066.400014
Produced by FastQC (version 0.11.5)