FastQCFastQC Report
Mon 27 Feb 2023
SRR3126389.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126389.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936732
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157970.8156523463236007No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25200.13011609246916972No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22770.11756918355249976No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC20180.10419614071539066TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG20110.10383470712519852No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGCTGTG19420.1002720045933046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55200.085.918621
AGGGATG38000.078.287485
ATAGCGG3850.074.547811
TAGGGCA11000.073.059324
TATAGGG17300.071.734982
GAATAGG10500.071.6962661
GGATGGC36250.071.565437
GTAGGGC11250.071.435783
AAGGGAT34650.071.072284
TAGCGGG9600.071.001892
ATAGGGC13000.070.857353
CGTAGGG5150.070.284032
ATAGGGA20550.070.209973
TAGGGCG3700.069.860744
TAAGGGA18100.069.84673
AGGGCAT17850.069.771795
ATAGAGG14750.069.53931
AGTAGGG34600.069.425322
GGTAAGG10850.069.383481
CGAAGGG20950.068.885452
Produced by FastQC (version 0.11.5)