FastQCFastQC Report
Mon 27 Feb 2023
SRR3126388.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126388.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986715
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158870.7996617531956018No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26040.13107063670430838No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG22870.11511464905635685No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22770.11461130559743095No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC22010.11078589530959398TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG20590.10363841819284597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58100.083.9631351
CGTAGGG6050.076.115932
AGGGATG36650.075.9018865
ATAGCGG4300.072.204041
TAAGGGA20750.070.428283
AGGGCAT17050.070.27825
CGAAGGG20250.070.078812
TAGGGCA10250.069.682634
ATAACGG2500.069.62221
TAGAGGG36700.069.14052
TATAGGG16450.069.1280442
GAGGGTA10950.069.0902254
ATGCGGG11200.068.8066942
ACGGGAT6700.068.7315444
GGTAAGG12550.068.5951461
ATAGAGG14800.068.338081
TAGGGTA7100.068.1684954
ATAGGGC13150.067.8941963
TAGCAGG7900.067.883431
AGTAGGG33850.067.882062
Produced by FastQC (version 0.11.5)