FastQCFastQC Report
Mon 27 Feb 2023
SRR3126386.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126386.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779173
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144570.8125685360557968No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACCCGTAA22540.12668807361622508No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG21920.12320330850344514No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21860.1228660731699503No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC20480.1151096604995692No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG19370.10887080682991479No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18620.1046553651612294No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53600.085.056291
GTAGGGC8200.075.082883
TAGGGCA9450.072.611354
AGTAGGG27500.072.1536942
CGTAGGG5200.070.529382
ACGGGTA2550.070.0368964
TAAGGGA13750.069.044983
TAGGGTC5750.068.658594
ATAGAGG11700.068.3613361
ATGAGGG27900.068.085712
ATAGGGC12250.067.907953
ATAGGGA13850.067.867783
GAATAGG11200.067.6324841
ACGGGAT3550.067.518884
AGAGGGC26350.067.420913
GTAAGGG29150.067.101712
AATAGGG25550.066.802772
AAGAGGG60500.066.4491652
TAGGGCG3050.066.260094
AGTAAGG12700.065.942251
Produced by FastQC (version 0.11.5)