FastQCFastQC Report
Mon 27 Feb 2023
SRR3126384.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126384.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030071
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182700.899968523268398No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG27450.13521694561421743No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25320.1247247017468847No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG24930.12280358667258436No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC24030.11837024419342969No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21240.10462688250805022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60100.085.306471
TAGGGCA11400.081.215364
GTAGGGC9850.073.955813
ATAGGGC13550.072.1441043
TAGGGTA8900.071.288684
AGTAGGG34150.070.875042
GTAGGGT8950.069.840193
TACGGGT2450.069.057883
AGAGGGC30400.068.641413
AATAGGG29850.068.489142
AGTAAGG11700.068.342841
TAGAGGG36300.068.101382
ACGGGTA2350.067.9967044
ATAGGGA17750.067.782633
ACGGGTC2850.067.610764
ATAGCGG3900.067.53881
CGTAGGG6200.067.464472
ACGGGAT3850.067.139614
TAAGGGA16250.066.8090743
AGGGCAT18750.066.67415
Produced by FastQC (version 0.11.5)