FastQCFastQC Report
Mon 27 Feb 2023
SRR3126383.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126383.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2243509
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300661.3401328008936002No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32950.14686814271750193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100600.086.021891
ATAGGGC12100.076.1262053
AGTAGGG35250.074.8224262
TAGACGG2800.070.5856551
TAGGGCA11100.070.282724
AGGGCAT17350.069.614085
GTAGGGA17400.068.603763
GGTAAGG12400.067.929211
ATAGCGG5750.067.92591
ACGGGAT5200.067.78314
ATAGGGA16700.067.5395663
AATAGGG29700.067.117762
AGGGATG27600.066.748345
GAATAGG15000.066.507371
AGGGACT31000.065.7947855
AGAGGGC35000.065.391973
ACGGGTA2300.065.386124
CGAAGGG24750.065.344832
AAGAGGG72400.065.1963352
AGATAGG13200.064.881761
Produced by FastQC (version 0.11.5)