FastQCFastQC Report
Mon 27 Feb 2023
SRR3126382.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126382.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2319472
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306461.3212489739044058No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31310.13498761787165356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106400.086.315381
GAATAGG15400.073.28551
ATAGCGG5900.072.529871
GGTAAGG12350.071.2036361
ATAGGGC13750.069.374913
AGTAGGG37150.068.936122
ATAAGGG30950.068.322062
AATAGGG35850.068.159012
AGGGATG30000.067.35295
CGTAGGG7000.067.1291352
ACGGGTA2600.066.870954
GTAGGGA16600.066.522553
AGGGACT29100.066.367885
AAGAGGG79700.065.739392
ATAGGGA18250.065.6578143
AGATAGG14200.065.569881
AGGGCTC25900.065.3148355
TAGGGCA12900.065.203734
TAAGAGG17250.065.153291
TAGAGGG46750.065.133222
Produced by FastQC (version 0.11.5)