FastQCFastQC Report
Mon 27 Feb 2023
SRR3126381.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126381.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2595090
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382681.4746309376553415No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35750.13776015475378503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT124500.086.73711
GAATAGG16950.074.364811
ATAGGGC15250.074.270223
ACGGGTA2350.073.994834
AGTAGGG40700.072.862232
GGTAAGG16550.069.625841
AGGGACT34400.069.265515
TAGGGCA12000.067.361954
ATAGAGG20200.067.2897341
AGGGATG33400.067.2587665
GTAGGGA18250.067.211743
TAAGGGA22150.066.835053
TAGAGGG52550.066.448262
AGAGGGC37450.066.3852163
ATAGGGA19950.066.195883
CGTAGGG7700.065.9174652
AATAGGG38450.065.880962
AGTTAGG7300.065.717291
GAGGGAT27900.065.6943364
TATAGGG19650.065.2931442
Produced by FastQC (version 0.11.5)