FastQCFastQC Report
Mon 27 Feb 2023
SRR3126380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2350592
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219920.9355940971465911No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG24700.10507991178392508No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC24370.10367601012851231No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24330.10350584023088652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76900.085.569691
CGTAGGG6550.077.5330662
ATAGGGC15200.075.133943
TAGGGCA14300.073.94694
AGGGATG31700.071.311465
ATAGGGA22050.070.5493243
AGTAGGG40450.070.0976262
ACGGGAT6900.069.474364
GGTAAGG13850.069.3297351
ATAGCGG5800.068.9810941
TAAGGGA23000.068.861353
AGTAAGG14500.068.818791
ATAAGGG34500.068.284662
AGAGGGC32350.068.2805563
GTACGGG5950.067.96512
AGGGCAT20300.067.3706055
AGGGACT32400.067.304865
GTAAGGG36550.067.156432
AATAGGG31800.066.836822
ACGGGTC3100.066.705934
Produced by FastQC (version 0.11.5)