FastQCFastQC Report
Mon 27 Feb 2023
SRR3126379.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126379.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372079
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222060.9361408283619558No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG26900.11340263119398637No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC25020.10547709414399774No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24040.10134569717113132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75750.086.124231
CGTAGGG6450.079.406982
ATAGGGC15100.073.751773
AGGGATG31750.073.555255
TAGGGCA12550.073.011614
AGTAGGG41150.071.481952
GTAGGGC11750.070.384313
TAAGGGA20950.070.203923
GAATAGG15250.070.014431
GGTAAGG13550.069.773141
ATAGGGA24500.069.045833
TAGGGTA7250.068.701934
ATAAGGG32500.067.663492
AATAGGG33800.067.563392
TAGAGGG42400.067.047322
TAGCGGG12700.066.967912
CGAAGGG22600.066.9482652
AGAGGGC33250.066.8452453
AGGGCAT19200.066.568495
GTAAGGG37500.066.535772
Produced by FastQC (version 0.11.5)