FastQCFastQC Report
Mon 27 Feb 2023
SRR3126378.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126378.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2663057
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281181.0558542306830083No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC28510.10705741559418368No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG28040.10529252659631394No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26870.10089907951651055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92200.084.731091
AGGGATG36700.074.403945
CGTAGGG9150.073.450152
ATAGGGC17750.073.079673
ATAGCGG6150.071.888061
TAGGGCA13850.071.6009144
AGTAGGG42650.070.634472
GGTAAGG14100.069.71581
ACGGGAT6850.069.297364
ATAGGGA26200.068.345443
TAGAGGG47450.068.2434162
GAATAGG16550.067.636791
AATAGGG39750.067.393112
GTACGGG6350.067.351212
TAGGGAT21100.067.268284
AGTAAGG16350.066.738171
AGTACGG3250.066.569991
ATAAGGG37550.066.4603042
GATAGGG40300.066.356722
CGAAGGG25750.066.0707242
Produced by FastQC (version 0.11.5)