FastQCFastQC Report
Mon 27 Feb 2023
SRR3126377.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126377.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192473
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193720.8835684635569059No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38250.1744605292744768No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG32690.14910103796033064No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC29030.13240755986504738TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG27910.12729917312550712No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26870.12255567115307692No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTACCAGTA25940.11831388573542298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66350.083.193361
ATAGCGG4750.078.264551
CGTAGGG5700.076.7206952
GTAGGGT9100.073.333723
TAGGGCG3350.072.948324
TAGGGTC6400.072.696254
AGTAGGG36100.072.161742
CGAAGGG21850.071.6633762
ATAACGG1450.071.398031
GTACGGG5850.069.9306952
TAAGGGA18550.069.92333
GTAGGGA16650.069.7171253
AGGGAAT26550.069.564045
AGAGGGC31450.069.185823
AGGGCAT19550.068.750535
TAGCGGG12350.068.5346152
TAGGGCA9550.068.401914
AAGGGTA16250.068.252014
ACGGGAT4850.067.828634
ATAGGGC12300.067.245673
Produced by FastQC (version 0.11.5)