FastQCFastQC Report
Mon 27 Feb 2023
SRR3126376.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126376.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262987
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194530.8596160737998053No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38990.17229440557988182No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG34220.15121606973438204No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC33720.1490066005681871TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG29020.12823759040595462No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26800.11842754730804907No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTACCAGTA24100.10649641381059635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64500.084.136011
ATAGCGG5000.075.241211
ATAGGGC15350.073.165883
GTAGGGA16650.072.815613
CGTAGGG7000.072.501142
TAAGGGA21400.072.0243153
AGAGGGC31750.071.930253
AGTAGGG32900.071.129952
AGTAAGG15550.070.765451
AGGGCAT19200.070.731965
ACGGGTA2750.070.060014
TAGGGCA10350.069.919684
AAGGGTA15050.069.628564
TAGGGCG4050.069.6174
CGAAGGG22350.069.173132
AGGGTAC12100.069.127955
AGGGAAT25150.068.945745
ATAGGGA19750.068.76223
ACGGGAT5200.068.679844
GGTAAGG12200.068.225891
Produced by FastQC (version 0.11.5)