FastQCFastQC Report
Mon 27 Feb 2023
SRR3126375.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126375.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2513198
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246500.9808220442639219No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46180.18374994727832825No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC38130.1517190448185937TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG36600.14563118385419693No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG33970.1351664293859855No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30320.12064310094150958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78550.085.4367141
ACGGGTA3300.082.604594
ATAGCGG5700.080.031991
CGTAGGG7600.074.2092062
TAGGGCG4500.074.1542364
AGAGGGC37450.071.659613
CGAAGGG24400.071.4618452
TAGGGCA11200.070.918384
ATAGGGC16750.070.709193
AAGGGTA19300.070.133474
AGGGATG34250.069.984165
AGTAAGG16200.069.963051
AGGGCAT24350.069.29245
AGTAGGG39550.069.2806852
AGGGTAC14850.068.995415
ATAGAGG16900.068.456571
GGTAAGG14100.068.3756561
AAGAGGG82200.068.154512
TAAGGGA22700.067.910693
AGATAGG14650.067.734751
Produced by FastQC (version 0.11.5)