FastQCFastQC Report
Mon 27 Feb 2023
SRR3126374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348335
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231870.987380420595869No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28880.12298075019109285No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC24030.10232781949764407Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86550.085.5352551
GGTAAGG13050.071.767211
CGTAGGG6300.070.15582
AGTAGGG37700.070.0924452
ATAGGGC13200.068.003653
GTAGGGC11500.067.430893
GGAGCTA44650.067.048769
CCGTACA11200.066.299743
ATAGCGG5400.066.237411
AGTAAGG14050.065.989281
TCCGTAC11650.065.786682
CGGGTAT1950.065.073165
AAGGGGC62700.064.986684
GTAGGGA18400.064.8766863
ATAAGGG31050.064.661012
GAATAGG13550.064.603671
TAGGGTA6500.064.350134
AGAAGGG81050.063.930022
TAGAGGG40250.063.782722
ATAGAGG16400.063.7078061
Produced by FastQC (version 0.11.5)