FastQCFastQC Report
Mon 27 Feb 2023
SRR3126373.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126373.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434713
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232900.9565809193937849No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28620.11754978923593869No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85150.084.543271
ATAGGGC14750.070.407543
AGTAGGG40700.070.3139952
TAGGGCA12950.069.307864
TAAGGGA20150.069.029633
CGTAGGG7900.069.0001142
GGAGCTA54500.068.9811259
ACGGGAT6000.068.920814
TAGTAGG10600.068.801531
AGGGAAT25050.068.2835
ATAAGGG31100.067.994072
GGTAAGG15750.067.514971
AGTTAGG6650.067.216231
ACTAGGG10350.067.195512
GTAGGGC12850.066.555993
AATAGGG33450.066.307832
AGTAAGG16050.066.253011
AAGGGGC67100.064.919654
GTAAGGG41400.064.811892
AGGGATG27000.064.569745
Produced by FastQC (version 0.11.5)