FastQCFastQC Report
Mon 27 Feb 2023
SRR3126372.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126372.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733025
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294661.0781460103731215No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31180.11408604019355842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105350.086.219521
GGTAAGG16150.074.2720641
AGTAGGG43500.071.847012
GACCGAT1450.071.30558
TAGAGGG46050.070.215842
ACGGGAT6050.069.137064
TAGGGTA6800.069.114214
ATAGGGC15950.068.9495543
CGTAGGG8250.068.929912
AGGGATG31450.067.39575
ACGGGTA3000.067.363324
AAGGGAC25750.067.3481144
ATAGGGA25050.066.978713
TATAGGG23950.066.915262
TAGTAGG11050.066.407971
GTACGGG5750.066.2054142
ATAAGGG36200.066.082362
GTAGGGA20850.066.0446853
GGAGCTA62200.065.88629
GTAGGGT9000.065.796733
Produced by FastQC (version 0.11.5)