FastQCFastQC Report
Mon 27 Feb 2023
SRR3126370.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126370.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478757
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121230.8198101513636114No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG37820.25575534046499865No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC35250.2383758792012481No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGTATTCA34960.23641477267732294No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG34310.2320191890892148No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24760.1674379225254724No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT16430.1111068282347945No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA15910.107590361364308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46100.084.688341
CGTAGGG3450.083.0866242
TAGGGCA8550.080.2457054
ATAGGGC8250.079.746043
GTAGGGC7900.079.115143
AGTACGG2300.073.624281
CGAAGGG14500.072.593942
AGTAGGG22450.071.7956542
GTAGGGT5850.071.4939353
TAGGGCG2250.071.0119554
TAGGGAC6950.070.320764
AAGGGAC10900.070.274254
ATAGGGA9650.070.124573
ACGGGAT3500.069.8184744
AATAGGG18500.069.598392
GCGTAGG2100.069.436661
AGGGATG14100.068.990025
GGTAAGG8050.068.949731
GTAGGGA10650.068.835113
GAATAGG7450.068.820311