FastQCFastQC Report
Mon 27 Feb 2023
SRR3126369.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126369.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636228
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156750.9579960738967919No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC40350.24660377404615982No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG39990.24440359167548775No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG38770.23694741808598804No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30050.18365411177415372No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT19270.11777087300791822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57150.084.414951
TAGGGCA9750.078.570374
ATAGGGC8500.077.407843
CGTAGGG3800.076.680332
GTAGGGC9350.071.878713
AGGGAAT20200.071.194415
ATAGCGG2650.070.9744951
GTACGGG3050.070.881652
AGGGTAC7200.070.496435
TAAGGGT6450.069.949943
AGGGCAT11800.069.3015755
AGTACGG1900.069.2935261
TACGGGT950.069.259653
TAGGGAC6450.069.22134
ATAGAGG10300.068.4766161
CGAAGGG14100.068.329872
AATAGGG22950.068.192622
ACGGGTA2000.068.1465454
TAGGGTC3450.068.112494
AAGGGTA11050.068.050854
Produced by FastQC (version 0.11.5)