FastQCFastQC Report
Mon 27 Feb 2023
SRR3126368.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126368.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056640
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232911.1324782168974639No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24340.11834837404698925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89900.087.1005861
AGTAGGG31900.071.9518362
ATAGCGG5450.071.708411
ATAGGGC11800.071.689353
ATGCGGG9100.070.292712
AGGGCAT16350.069.560355
GGTAAGG12250.069.1870651
GTACGGG4700.069.050042
TAGGGCA10200.067.269294
AGTAAGG12600.067.26521
AGATAGG12650.066.999341
TAAGGGA17100.066.7842943
CGTAGGG5750.066.2567142
AGGGATG27550.066.0166555
GTAAGGG33550.065.889782
GTAGGGA14850.065.1935963
GTAGGGC10700.065.0042953
TAGGGAT11300.064.464034
AGAGGGC28550.064.198173
AAGAGGG68150.063.9083862
Produced by FastQC (version 0.11.5)