FastQCFastQC Report
Mon 27 Feb 2023
SRR3126367.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126367.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120133
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235241.1095530327578504No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22880.10791775798971104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89450.087.506731
TAAGGGA17400.071.8406753
ATAGGGC10400.071.3940053
ATGCGGG10700.070.703222
ATAGCGG5800.070.560191
GGTAAGG12550.069.342021
GTAAGGG34300.068.634212
AGGGATG29000.068.545735
AGGGCAT17350.068.526585
TAGGGCA9150.067.280354
AGTAGGG33800.067.008122
TATAGGG17600.065.9449162
ATAGGGA17150.065.763533
GTAGGGA14750.065.631643
AAGAGGG71900.065.614812
GAATAGG13200.065.571081
ACGGGAT6250.065.4149864
AGAGGGC31050.065.07963
AAGGGGC58300.065.049364
AGGGAAT21750.065.034725
Produced by FastQC (version 0.11.5)