FastQCFastQC Report
Mon 27 Feb 2023
SRR3126366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397460
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289621.2080284968258073No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26950.11241063458827258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109400.086.296061
ATAGGGC12750.072.246563
GTAGGGA17900.071.939733
ACGGGAT5050.071.658974
TAGGGTA8650.070.088194
ATAGCGG6000.068.990991
TAGGGCA10800.068.755044
TAAGGGA20850.068.748793
AGTAGGG40100.068.6790852
AGTACGG3450.068.1729051
GAATAGG15700.067.4130251
TAGGGAT16900.066.741484
TAGAGGG42950.066.6385352
ATGCGGG11850.066.628832
AGGGATG34100.066.567775
CGTAGGG6850.066.550672
GTACGGG6200.065.947572
ATAAGGG33650.065.7820052
ATAGGGA20900.065.661063
TGTAGGG18250.065.1521762
Produced by FastQC (version 0.11.5)