FastQCFastQC Report
Mon 27 Feb 2023
SRR3126365.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126365.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450392
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129270.8912762894445089No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26060.17967556357177922No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG24320.1676788068329114No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCTACGCA21490.14816684041279873No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC20580.14189267453212648No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG19600.13513588050678713No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18260.1258969988803027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT44350.084.057161
GAATAGG7350.074.9275741
AGGGAAT16100.074.141565
ATAGAGG8200.072.9009251
AGGGATG22350.071.912185
ATAAGGG17250.071.700152
AGTAGGG23250.071.603422
ATGAGGG21100.071.544462
TAGGGCA6250.071.432774
ACGGGAT3900.071.095374
TAAGGGA12300.071.065983
TACGGGT605.456968E-1270.492873
TGTAGGG10000.069.130682
CGTAGGG3200.069.0718842
GGTAAGG8050.068.996851
GTACGGG2550.068.236232
ACGGGTC2000.068.1431054
AGGGCAT13050.067.701965
CGAAGGG14750.067.592312
GTAGGGA10650.067.51433
Produced by FastQC (version 0.11.5)