FastQCFastQC Report
Mon 27 Feb 2023
SRR3126364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505217
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131020.8704392788548097No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26010.17279900506039994No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG25830.17160316419493005No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC21880.14536110075822956No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCTACGCA21290.14144140014363377No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG20110.13360199891444224No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18880.1254304196670646No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT47000.085.050571
ACGGGTA1700.082.929864
TAGGGTA4200.074.9659654
AGGGAAT14900.074.748195
AGGGATG22650.074.276855
ATAGCGG3850.073.290331
TAGGGCA6350.072.5255364
AGTAAGG8450.071.794161
CGTAGGG3550.071.4784552
ATAAGGG20250.071.471922
AGGGCAT13000.071.213365
TAGACGG1850.071.177451
AGTAGGG23250.071.1425552
TAAGGGA11550.070.795533
TAGGGCG1800.070.490394
TACGGGT1000.070.490383
GAATAGG8250.070.114411
GTAGGGT5700.069.253713
AAGGGAC13650.069.199354
ATGAGGG22200.068.580682
Produced by FastQC (version 0.11.5)