FastQCFastQC Report
Mon 27 Feb 2023
SRR3126363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676210
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166660.9942668281420587No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29460.1757536346877778No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG28080.1675207760364155No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG23980.14306083366642602No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC23900.14258356649823112No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22910.13667738529181905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58250.082.601871
AGGGATG25550.076.15455
GAATAGG8450.074.586161
ACGGGTA1450.074.549714
AGGGAAT17500.073.5865865
TAGGGTA4550.073.3386844
AGTAGGG25750.073.007742
AAGGGAC16550.072.130984
AGTACGG2700.071.4217151
GTACGGG4150.071.347472
GTAGGGA12500.070.686093
GTAAAGG12700.069.995191
AGAGGGC23150.069.838293
ACGGGAT6200.069.740054
ATAAGGG21650.069.466952
GAGGGAT18700.069.367114
AGGGCAT15450.069.357355
AGACGGG11600.069.2826162
ATAGAGG10050.069.263721
AGGGACT24700.068.5001755
Produced by FastQC (version 0.11.5)