FastQCFastQC Report
Mon 27 Feb 2023
SRR3126362.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126362.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035214
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145000.7124557908898033No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG28820.14160673029961468No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC27790.13654583744019058No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG23860.11723582876297038No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23770.11679361482379741No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCTGTAT20950.10293757806304397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59150.080.924771
CGTAGGG5750.077.697642
TAGGGCA13400.073.302044
ATAGGGC14150.072.738153
AGTAGGG32400.071.267012
ATAGCGG4850.070.84291
TAGCGGG10500.070.765222
ATAAGGG28750.070.663962
TAAGGGC20050.070.554823
ATAGAGG15200.069.671341
AGGGATG24300.069.62595
TGTAGGG14600.069.574962
CGAAGGG17300.069.318022
GTAGGGT8550.068.709773
AGGGCAT19100.068.404725
GTAGGGC12350.068.117813
GAAGGGC30150.067.963193
TAAGGGA15700.067.9517753
GTAGGGA14250.067.9402163
TAAGCGG5000.067.776281
Produced by FastQC (version 0.11.5)