FastQCFastQC Report
Mon 27 Feb 2023
SRR3126361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2065049
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144480.699644415217266No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG31760.15379780334510224No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC31610.15307142832930357No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG25280.12241840266260025No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22860.11069955240771527No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT22590.10939207737927768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60150.079.916941
CGTAGGG6650.078.435852
TAGGGCA13000.076.631234
ATAGGGC15700.076.023413
AGGGCAT18150.073.7872855
GAATAGG12250.072.184731
ATAGCGG4950.071.265551
AAGGGTC11650.070.587114
TAGCGGG13850.070.571092
ATAGGGA18800.069.236513
ACGGGTA1700.069.104194
AGTAGGG33000.068.635122
AGGGATG24850.068.453475
TAGAGGG38050.068.170692
GTAGGGC11050.068.0410463
AGGGCAC17200.068.027455
GGTAAGG12700.067.775221
AGTAAGG12650.067.671291
AGAGGGC30600.067.414983
CGAAGGG18300.067.276512
Produced by FastQC (version 0.11.5)