FastQCFastQC Report
Mon 27 Feb 2023
SRR3126360.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126360.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295196
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185660.808906951737455No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC34490.1502703908511517No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG33660.14665414195563253No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG28950.12613301870515634No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25370.11053522226424235No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT24540.10691897336872319No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG5200.082.251732
CGTTTTT72200.081.697381
ACGGGTA1950.079.540144
TAGGGCA13550.078.39284
ATAGCGG4700.076.0612641
GTAGGGC12400.075.429013
ATAGGGC15250.073.660573
ACGGGAT5900.072.493064
AGTAGGG35150.071.8052142
GTAGGGT8700.071.3118443
AGGGCAT20150.071.142945
TAGCGGG12700.070.3164442
AGACGGG13200.069.789352
GAATAGG14100.069.7228241
ATTACGG2300.069.534261
ATAGAGG16250.069.471341
ATGAGGG33300.069.3017652
ATAGGGA19200.068.543113
CGAAGGG21750.068.5025942
AGGGCAC19800.068.12775
Produced by FastQC (version 0.11.5)