FastQCFastQC Report
Mon 27 Feb 2023
SRR3126359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003916
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159280.7948436960431475No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28490.14217162795246907No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC25440.12695142910181864No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG24830.12390738933168856No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23910.11931637853083663No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCGCTAAAT23530.11742009146091953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55250.084.998571
CGTAGGG5100.081.127482
ATAGGGC14300.077.233033
ACGGGTA2800.077.209554
AGTAGGG28800.074.443682
GAATAGG11900.073.944861
GTACGGG3800.073.0001752
GTAGGGC9600.072.943443
TAGGGCA11850.072.181224
ACGGGAT6550.071.033814
AGGGATG33150.070.8855445
ATAACGG1600.070.583731
TAAGGGA17800.069.967623
AGAGGGC27900.068.726973
AGGGCAT15900.067.983255
GAGGGAT28000.067.306594
ATGCGCG353.6408746E-667.138749
CGAAGGG20250.067.100892
TAGGGTC6250.066.92394
ACTAGGG9600.066.6075062
Produced by FastQC (version 0.11.5)