FastQCFastQC Report
Mon 27 Feb 2023
SRR3126358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041778
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158600.7767739685705302No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28260.13840877901515247No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG27370.13404983303767598No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC27170.13307029461577116No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23890.11700586449653196No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCGCTAAAT23260.11392031846753173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54650.084.350781
GTACGGG3550.076.7706452
TAGGGTC6350.074.73834
TAGGGCA13150.074.682024
ACGGGTA2650.074.473034
ATAGGGC15450.072.384243
TAAGGGA18350.071.4436653
AGGGATG31350.071.195355
AGAGGGC28650.071.0164263
AGTAGGG32250.070.519822
GAGGGAT27500.069.885354
AAGAGGG69200.069.532752
ATAGCGG3050.069.401011
AGGGCAC17300.068.717925
CGGGTAT2950.068.492355
GTAGGGA15150.068.234753
TAGGGCG3650.068.230514
CGTAGGG5600.067.966132
AGAAGGG68850.067.361042
ATGAGGG31800.066.937062
Produced by FastQC (version 0.11.5)