FastQCFastQC Report
Mon 27 Feb 2023
SRR3126356.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126356.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379514
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114450.8296400036534606No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28070.20347745655353985No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCGTTGA27270.1976783127971155No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC24380.1767289059770325No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG22800.16527559705809436No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG21790.1579541780656086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT42450.083.7213441
ACGGGTA1550.081.863464
CGTAGGG2750.078.659432
GTACGGG2250.077.3294452
TAGGGCA8200.076.224724
TATAGGG10500.076.135172
CTACGAC4700.072.99335
AGGGATG19650.071.9883045
TAAGGGA11100.071.975373
ACGGGAT4450.071.813634
TAGCGGG7350.071.016832
GTAGGGA10400.070.493533
GGTAAGG8600.069.5139851
ATGCGGG7800.069.331232
AGGGCAT12600.069.00165
ATAGGGC8200.068.774183
AGTAGGG21950.068.769552
TAGAGGG22200.068.6305852
AGAGGGC19450.068.379333
CGAAGGG12750.068.2318652
Produced by FastQC (version 0.11.5)