FastQCFastQC Report
Mon 27 Feb 2023
SRR3126355.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126355.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417706
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114290.80616150316074No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30410.2145014551677146No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCGTTGA27510.19404587410930052No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC24850.17528316872468622No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG24030.1694991768392036No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG22860.16124640792942965No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41150.083.4407041
TAGGGCA9700.079.929684
ACGGGTA1500.078.314934
CGTAGGG3350.075.74342
GTACGGG2400.074.3991852
AGAGGGC19000.072.214613
CTACGAC5950.071.865475
ATAGGGC9900.071.670033
TATAGGG9800.071.4424062
AGGGAAT17250.071.368745
AGGGCAA10600.070.4834445
AAGGGCA19950.070.424564
AGGGATG22700.069.137955
TAAGGGA12450.068.690693
AGTAGGG22600.068.404282
AGGGCAT12500.068.0400165
TAGCGGG5950.067.916822
ATGCGGG6950.067.6100162
AAGAGGG43250.067.35992
ATAGGGA13450.067.077183
Produced by FastQC (version 0.11.5)