FastQCFastQC Report
Mon 27 Feb 2023
SRR3126354.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126354.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601993
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142800.8913896627513354No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34500.21535674625294868No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG28290.17659253192741792No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC26920.16804068432259067No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG24990.15599319098148368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54150.084.4075241
CGTAGGG4000.079.897252
TAGGGCA10000.075.667384
CTACGAC6750.075.19745
TAGACGG2900.074.588731
GTACGGG2900.074.549152
ATAGGGC10200.074.1837163
ACGGGTA1650.074.058054
ACGGGAT5200.072.30524
AGGGATG23900.071.382475
TATAGGG12150.070.787682
AGAGGGC22200.070.709273
ATAGCGG3600.070.5349961
GACCGAT1000.070.495378
CGAAGGG12450.069.8369452
TAGAGGG26100.069.507182
AGGGCTC15200.068.951575
TACGGGG7750.068.5266653
AAGGGCA22400.068.189614
TAGCGGG8700.068.066612
Produced by FastQC (version 0.11.5)