FastQCFastQC Report
Mon 27 Feb 2023
SRR3126353.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126353.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989324
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87190.8813088533180233No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGACGTGC38860.39279346301110657No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC20180.20397766555749178Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG19430.19639673150555328TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG19250.19457730733308803TruSeq Adapter, Index 22 (95% over 23bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA13460.13605249645212286No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATG11330.11452264374461753No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC10640.10754818441683413No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT10040.10148343717528333No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT37350.085.8136441
ACGGGTA1100.085.4476554
ATAGCGG1850.076.321941
CGGGTAT1050.076.0890965
AGGGATG16750.076.035665
GAATAGG5950.075.14611
AGGGAAT12850.074.6087655
AAGGGCG4600.073.559294
GTACGGG2200.072.674582
GGGCGAT2850.072.555556
TAGAGGG15950.072.2323462
TAAGGGA8300.071.909863
GACCGAT850.071.876568
TAGGGTC2550.071.876564
TAGGGCA4700.070.994274
AGGGCAT10650.070.604645
AGGGTAC4400.070.4943165
TACGGGT605.456968E-1270.4943163
GTAGGGA8350.070.353613
GCATAGG3350.070.246561
Produced by FastQC (version 0.11.5)