FastQCFastQC Report
Mon 27 Feb 2023
SRR3126352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056347
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90170.853602083406305No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGACGTGC37530.3552809824801888No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC21420.2027742777704675Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG20690.19586366979789785TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG20000.18933172527588TruSeq Adapter, Index 22 (95% over 23bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA13330.12618959489637402No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC12130.1148296913798212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT36500.084.3948751
GACCGAT850.077.40128
AAGGGCG3900.077.1176764
CTAACGG1050.076.142521
ACGGGTA1050.076.084854
GGTAAGG5800.075.40891
AGGGATG18650.074.836975
AGGGAAT13800.073.5551765
ACGGGAT2200.072.626464
TAAGGGA9650.072.560043
GAGGGAT15150.071.033214
TAGGGTC3450.070.830914
GAATAGG5650.070.751911
CGTAGGG4000.070.4870452
AGGGCAT10650.070.159435
ATAAGGG15050.069.940632
GCATAGG3500.069.871961
TAACGGG3700.069.852032
TAGGGCA6250.069.174564
AGAGGGC16050.069.09963
Produced by FastQC (version 0.11.5)