FastQCFastQC Report
Mon 27 Feb 2023
SRR3126351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191181
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115860.9726481533872686No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG21940.18418695395578003TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC21630.1815844947157485Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG21620.18150054441768296TruSeq Adapter, Index 22 (95% over 23bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGACGTGC16610.13944144508685077No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC13640.11450820656138738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT42650.084.373711
ATAGCGG1900.079.22481
CGTAGGG4200.078.327142
TAGGGCA6900.075.602724
AGGGAAT16200.075.426135
AGGGATG20700.075.375695
GGTAAGG6150.074.192741
TAGGGTC2800.073.851314
GGATGGC17750.072.546387
AGTACGG1500.072.127591
ACGGGAT3100.071.252434
CGAAGGG12750.071.139472
ATAGGGA10400.070.946313
AAGGGCG5700.070.906684
GTAGGGC6500.070.855943
AGGGCAC7700.070.79965
AAGGGTA7050.070.661094
TCGAGGG6400.070.494422
GAGGGAT16950.069.870584
AAGGGTC7550.069.716354
Produced by FastQC (version 0.11.5)