FastQCFastQC Report
Mon 27 Feb 2023
SRR3126350.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126350.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762255
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143430.8139003719665996No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30950.17562725031280946No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACGAGTCT29760.16887453858834278No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23780.1349407435359809No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG18960.10758942377805708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49150.082.847221
AGGGATG29350.077.979175
AGTAGGG26500.073.6408542
GAATAGG11650.073.541221
ATAGCGG4550.073.456851
TAGGGCA10200.073.2579354
GTAGGGC10150.072.229783
ATAGAGG12000.071.788551
ACGGGTA2100.071.6124344
CGTAGGG4600.071.557772
AGTAAGG10550.070.500141
GAGGGAT20550.070.207634
ATAGGGA15600.069.890983
AGATAGG9500.069.868411
TAGGGAT11250.069.762444
AGAGGGC24600.069.538293
TAGCGGG11050.068.939692
GGTAAGG11350.067.189981
AAGAGGG54800.067.188942
AGGGACT25650.066.8749165
Produced by FastQC (version 0.11.5)