FastQCFastQC Report
Mon 27 Feb 2023
SRR3126349.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126349.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809239
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146460.8095116233952507No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30200.16692100933044227No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACGAGTCT27780.15354521984104919No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24040.13287354517562355No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG20820.11507600709469562No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG19170.1059561506246549No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC18870.10429799490282932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49850.081.1447141
GTACGGG4750.076.1734852
AGTAGGG28900.075.119172
AGGGATG29950.074.6821445
ATAGGGA16600.071.0513463
AGAGGGC26400.070.841193
TAGGGCA8850.070.617944
GAATAGG11400.070.140851
ATAGAGG11900.069.1700441
ATAAGGG26250.068.381832
GTAAGGG28750.067.992682
AAGAGGG60300.067.874642
TCCGCTA6450.067.818821
TAGGGTA5000.067.6657944
GTAGGGC8800.067.281333
TAGAAGG14500.067.1474151
ACGGGTA2100.067.128764
TAGGGAT11850.067.0154654
TAAGGGA14400.066.569363
AGTAAGG11450.066.5482251
Produced by FastQC (version 0.11.5)