FastQCFastQC Report
Mon 27 Feb 2023
SRR3126348.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126348.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019671
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182630.9042561882603651No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34880.17270139542529453No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27230.13482393914652438No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG23520.11645461067668941No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG21860.10823545022927No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC21230.10511613030043013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57600.082.1609951
AGGGATG32600.075.108795
AGTAGGG31100.074.349032
ATAGGGA20200.073.75283
ATAGAGG13050.072.81671
CGTAGGG6200.072.769642
AAGGGTA12100.071.8554
GGTAAGG13400.071.6169051
GAATAGG13950.071.153851
TAGGGCA10000.070.4955754
AGGGAAT20000.070.4955755
ACGGGAT4750.070.248234
GAGGGAT24750.070.068334
AGAGGGC28500.069.7535253
TAGGGTA6500.069.411034
TAGACGG2450.069.123641
TAGAGGG36300.068.877232
TATAGGG17350.067.448222
TAATAGG9000.067.427551
AGATAGG11800.067.37441
Produced by FastQC (version 0.11.5)