FastQCFastQC Report
Mon 27 Feb 2023
SRR3126347.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126347.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739622
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152320.8755925137759811No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47210.27138079421851413No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATTAGACG24910.14319202677363244No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24860.14290460801254526No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG22000.12646425487835863No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC18540.10657487661112587No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG18230.10479288029238536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54700.085.6043851
CGTAGGG5500.080.373532
TAGGGTA5000.077.0756154
AGGGATG34000.076.9926765
AGTACGG3650.074.781711
AGTAGGG26300.072.775722
ACGGGAT5500.072.6322254
AGGGTAT8300.072.47785
TAGGGCA9600.072.4542164
GAATAGG10150.071.402751
ACGGGTA2450.070.9755554
CTACGAC9850.070.615275
AGACGGG13350.070.1002662
GTAGGGT6400.069.761663
AAGGGCG7900.069.603644
GGATGGC32750.069.455597
AGGGCAT15450.069.051095
AAGGGAC19550.068.993524
TACGACC9950.068.96096
GGTAAGG9500.068.3621441
Produced by FastQC (version 0.11.5)