FastQCFastQC Report
Mon 27 Feb 2023
SRR3126346.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126346.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785837
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156980.8790275932237936No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47180.26418984487386027No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23960.13416678005887434No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG23140.12957509559943042No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATTAGACG21540.12061571128832026No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC20570.11518408454970974No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG20320.11378418075109879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54800.085.753621
CGTAGGG5150.075.7335362
AGGGATG35650.075.664835
TAGGGCA10250.073.814854
AGGGAAT21200.072.707585
AGTAGGG30000.071.740042
TAAGGGA17850.071.083253
GGTAAGG11150.070.876451
TAGGGTA6500.070.852374
GTAGGGC10350.070.377373
ACGGGAT5100.070.030164
ATAGGGA16300.069.770123
ATAGCGG3850.069.643681
ACGGGTC2100.069.371994
AAGGGTA10850.069.299794
AGTAAGG11700.068.75081
ATAGAGG11850.068.674471
TAGTCGG551.6552804E-1068.421851
CTACGAC8250.068.35485
AAGGGAC18500.068.077684
Produced by FastQC (version 0.11.5)