FastQCFastQC Report
Mon 27 Feb 2023
SRR3126345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986825
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190960.9611314534495992No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54440.27400500799013505No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26840.1350899047475243No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG24400.12280900431593118No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG23700.11928579517572005No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC23310.117322864369031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65450.084.5799561
TACGGGT1750.083.25653
CGTAGGG6800.082.249362
AGGGATG42600.077.00885
TAAGGGA19400.073.406653
CTAACGG1350.073.16211
GTAGGGC10350.073.110553
AGGGAAT20700.072.656455
TAGCGGG11000.072.2085342
GGTAAGG10300.072.147371
ACGGGAT5800.072.120134
ACGGGTA2350.071.999444
TAGAGGG33550.071.164872
AGTAGGG31700.070.5735862
CTACGAC10300.070.27135
TACGACC10300.070.269526
ACGGGTC2550.070.038674
TAGGGCA13100.069.9612964
TAGGGTA6200.069.741394
GGATGGC37650.068.7812657
Produced by FastQC (version 0.11.5)