FastQCFastQC Report
Sat 18 Jun 2016
SRR3554444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359891
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33890.9416740068520746No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTC14650.40706769549669203No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGC13740.38178226185150504No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCG12190.33871366608223047No Hit
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC9730.27035963666776885No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5780.1606041829331659No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCACGATT4850.13476302547160113No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC4460.12392641105223527No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC3910.10864400610184752No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT3710.10308676793807015No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA253.885446E-545.00000423
AGGGCGC253.885446E-545.0000046
AGTACGG502.1827873E-1145.0000042
CGACGTT207.026821E-445.027
GCACGGT207.026821E-445.021
CCTACCG207.026821E-445.014
GCGTTAG207.026821E-445.01
GTAAGCG207.026821E-445.01
CTCTCTA207.026821E-445.039
TCGACGT207.026821E-445.026
ATCGAGG207.026821E-445.02
AATCGCG207.026821E-445.01
CACGTGA302.1613305E-644.99999643
AACACGT302.1613305E-644.99999641
CGTTTTT16650.042.70271
AAGGGCG750.042.05
GTACGGG1200.041.2499963
CACTAGG556.002665E-1140.909092
TGCACGG1050.040.7142832
TAATACG501.0768417E-940.5000044