FastQCFastQC Report
Sat 18 Jun 2016
SRR3554441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289880
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45691.5761694494273493No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTC23150.7986063198564923No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCG22780.7858424175520904No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGC21340.7361666896646888No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC13780.47536911825583No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC7630.26321236373671864No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTTCGGAT7600.26217745273906445No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCT5600.19318338622878434No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT5570.1921484752311301Illumina Single End Adapter 1 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.15558161998068168No Hit
GAACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT4450.15351179798537326No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTC4120.14212777701117704No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTA4100.14143783634607424No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4030.13902304401821444No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC3430.1183248240651304No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCC207.0245506E-445.034
TACCACG207.0245506E-445.01
TAGACGG207.0245506E-445.02
GCGAACC207.0245506E-445.033
CGCATGG253.883565E-545.02
GGCGTAG207.0245506E-445.01
TAACGTA253.883565E-545.032
TCTAGCG207.0245506E-445.01
ATTCCGC207.0245506E-445.044
ACGATAG207.0245506E-445.018
AGTGATT351.207718E-745.042
CGTAGAA207.0245506E-445.035
CTCATGT253.883565E-545.015
GCGATTT207.0245506E-445.09
ACGTAGA207.0245506E-445.034
ATACGCA207.0245506E-445.041
CGATCGA453.8198777E-1045.041
GCATGAC207.0245506E-445.022
GTTGGTC207.0245506E-445.011
GACCTTA207.0245506E-445.09