##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554441_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	289880
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.954626052159515	33.0	31.0	34.0	30.0	34.0
2	32.01799365254588	33.0	31.0	34.0	30.0	34.0
3	31.99100317372706	33.0	31.0	34.0	30.0	34.0
4	35.691044570166966	37.0	35.0	37.0	33.0	37.0
5	32.54071684835104	37.0	35.0	37.0	25.0	37.0
6	34.023113012280945	37.0	35.0	37.0	25.0	37.0
7	35.48290327031875	37.0	35.0	37.0	32.0	37.0
8	35.48818821581344	37.0	35.0	37.0	33.0	37.0
9	37.428825720987994	39.0	37.0	39.0	34.0	39.0
10	37.149965502966744	39.0	37.0	39.0	34.0	39.0
11	37.189668138540085	39.0	37.0	39.0	34.0	39.0
12	37.13064371464054	39.0	37.0	39.0	34.0	39.0
13	37.0478232372016	39.0	37.0	39.0	33.0	39.0
14	38.16029391472333	40.0	37.0	41.0	33.0	41.0
15	38.324210017938455	40.0	37.0	41.0	33.0	41.0
16	38.17324755071064	40.0	37.0	41.0	33.0	41.0
17	38.15577480336691	40.0	37.0	41.0	34.0	41.0
18	38.00519525320822	39.0	37.0	41.0	34.0	41.0
19	37.76734165861736	39.0	37.0	41.0	34.0	41.0
20	37.65243894025114	39.0	35.0	41.0	34.0	41.0
21	37.63071270870705	39.0	35.0	41.0	33.0	41.0
22	37.75312888091624	39.0	35.0	41.0	34.0	41.0
23	37.720774113426245	39.0	35.0	41.0	34.0	41.0
24	37.62684904098248	39.0	35.0	41.0	33.0	41.0
25	37.41659997240237	39.0	35.0	41.0	33.0	41.0
26	37.49032703187526	39.0	35.0	41.0	33.0	41.0
27	37.45731337105009	39.0	35.0	41.0	33.0	41.0
28	37.327680419483926	39.0	35.0	41.0	33.0	41.0
29	37.34489443907824	39.0	35.0	41.0	33.0	41.0
30	37.04049951704153	39.0	35.0	41.0	33.0	41.0
31	36.862139506002485	39.0	35.0	41.0	32.0	41.0
32	36.52782875672692	39.0	35.0	41.0	31.0	41.0
33	36.18679453566993	39.0	35.0	41.0	30.0	41.0
34	35.887929488064024	39.0	35.0	41.0	27.0	41.0
35	35.74092038084725	39.0	35.0	41.0	25.0	41.0
36	35.532171933213746	39.0	35.0	41.0	24.0	41.0
37	35.46651717952256	39.0	35.0	41.0	23.0	41.0
38	35.38406927004278	39.0	35.0	41.0	23.0	41.0
39	35.27137091210156	39.0	35.0	41.0	23.0	41.0
40	35.184559127915	39.0	35.0	41.0	22.0	41.0
41	35.0218366220505	39.0	35.0	41.0	21.0	41.0
42	35.02474817165724	39.0	35.0	41.0	21.0	41.0
43	34.93773285497447	39.0	35.0	41.0	21.0	41.0
44	34.888039878570446	39.0	35.0	41.0	21.0	41.0
45	34.84693321374362	38.0	35.0	41.0	21.0	41.0
46	34.73106457844625	38.0	35.0	41.0	20.0	41.0
47	34.62862908789844	38.0	34.0	40.0	20.0	41.0
48	34.613267558989925	38.0	34.0	40.0	20.0	41.0
49	34.5716606871809	38.0	34.0	40.0	20.0	41.0
50	34.45095211811784	38.0	34.0	40.0	20.0	41.0
51	33.52847385124879	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	3.0
12	1.0
13	6.0
14	9.0
15	18.0
16	22.0
17	55.0
18	114.0
19	219.0
20	398.0
21	692.0
22	1260.0
23	1968.0
24	2824.0
25	4174.0
26	5859.0
27	6223.0
28	5726.0
29	5253.0
30	4939.0
31	5924.0
32	7251.0
33	10380.0
34	18242.0
35	23437.0
36	21197.0
37	30525.0
38	51589.0
39	81520.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68766386090796	16.00696840071754	19.77369946184628	21.53166827652822
2	37.45791361942873	25.139023044018217	21.595487788050228	15.807575548502829
3	28.137850144887537	24.215537463778112	33.09541879398372	14.551193597350629
4	25.86622050503657	20.735476748999588	36.5026907685939	16.895611977369946
5	23.414861321926313	31.55133158548365	28.461777287153307	16.572029805436735
6	22.494825445011728	32.34993790534014	31.392990202842558	13.762246446805577
7	68.45556782116738	5.4539809576376435	21.52442389954464	4.5660273216503375
8	68.16165309783358	8.657030495377398	18.060921760728576	5.120394646060439
9	63.52490685801021	6.345039326617911	20.06623430384987	10.06381951152201
10	37.708017110528495	22.028425555402237	26.95356699323858	13.309990340830687
11	28.882641092866013	21.493721539947565	32.86842831516489	16.755209052021527
12	25.713398647716296	19.674692976404028	35.53918862977784	19.072719746101836
13	22.95018628397958	23.03642886711743	37.41168759486684	16.601697254036154
14	18.087829446667588	26.372981923554573	35.43328273768456	20.10590589209328
15	16.63101973230302	22.493445563681526	41.46060438802263	19.414930315992823
16	19.147923278598043	23.079550158686352	36.43507658341383	21.33744997930178
17	19.227956395749963	24.198633917483097	35.44570166965641	21.12770801711053
18	19.939285221470954	22.543121291568923	36.91975990064854	20.59783358631158
19	19.932385814819924	24.463916103215126	34.622947426521314	20.980750655443632
20	22.682489305919688	24.304884779908928	36.6872498965089	16.32537601766248
21	20.345660273216502	26.79074099627432	35.85173175106941	17.01186697943977
22	19.929626052159513	21.62963985097282	36.35849316958741	22.082240927280253
23	18.87194701255692	24.61397819787498	36.21981509590175	20.294259693666344
24	19.712639712984682	22.970884503932663	37.00876224644681	20.30771353663585
25	17.94121705533324	26.406444045812062	34.93307575548503	20.71926314336967
26	17.346143231682078	24.540154546708983	36.993928522147094	21.119773699461845
27	20.75651993928522	24.111701393680143	35.986960121429554	19.144818545605077
28	16.092866013522837	24.24417000137988	38.72602456188768	20.936939423209605
29	18.716020422243687	22.127777011177038	38.756036980819644	20.400165585759623
30	20.452256105974886	24.489098937491377	35.99972402373396	19.05892093279978
31	21.448875396715884	22.923968538705672	34.65744445977646	20.969711604801986
32	22.572098799503244	24.158617358907133	34.7167793569753	18.552504484614325
33	21.63274458396578	23.444873740858284	31.71415758244791	23.208224092728024
34	20.626121153580794	23.235131778667036	35.056575134538434	21.082171933213743
35	22.513108872636952	23.87746653787774	32.418242031185315	21.191182558299985
36	21.582033945080724	27.145025527804613	32.36097695598178	18.911963571132883
37	21.781081826962883	25.511591003173727	33.929557058093	18.777770111770387
38	20.407064992410653	25.798606319856493	31.525803780874845	22.268524906858012
39	22.100179384572925	24.811646198426935	32.03049537739754	21.057679039602593
40	24.031323306195667	23.127501034910996	31.518559403891267	21.322616255002067
41	21.826617910859667	24.994135504346627	29.954118945770663	23.225127639023043
42	22.062577618324823	24.417000137988133	32.135366358493165	21.385055885193875
43	22.27335449151373	24.80957637643163	31.09183110252518	21.825238029529462
44	20.595418793983715	24.053056437146406	32.21988408996827	23.131640678901615
45	20.60024837863944	22.992272664550846	31.72968124741272	24.67779770939699
46	22.662825996964262	24.990340830688563	31.58341382641093	20.76341934593625
47	19.718849178970608	23.572167793569754	35.60576790396026	21.103215123499382
48	20.839657789430106	23.994066510280117	32.934662619014766	22.231613081275007
49	21.040085552642473	22.526562715606456	34.7543811232234	21.678970608527667
50	19.99068580102111	23.229957223678763	33.42866013522837	23.350696840071755
51	20.37946736580654	22.195391196357114	31.39885469849593	26.026286739340414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	153.0
1	418.0
2	683.0
3	4833.5
4	8984.0
5	5987.5
6	2991.0
7	2968.5
8	2946.0
9	2859.0
10	2772.0
11	2707.0
12	2642.0
13	2535.0
14	2428.0
15	2312.0
16	2196.0
17	2095.5
18	1995.0
19	1840.5
20	1686.0
21	1768.0
22	1850.0
23	1920.5
24	1991.0
25	2039.0
26	2477.5
27	2868.0
28	3328.0
29	3788.0
30	4184.5
31	4581.0
32	5208.5
33	5836.0
34	6582.0
35	7328.0
36	7980.0
37	8632.0
38	9051.5
39	9471.0
40	10622.5
41	11774.0
42	13610.5
43	15447.0
44	17067.5
45	18688.0
46	24349.0
47	30010.0
48	28077.0
49	26144.0
50	25931.0
51	25718.0
52	22556.5
53	19395.0
54	17373.0
55	15351.0
56	13622.5
57	11894.0
58	10695.0
59	9496.0
60	8750.0
61	8004.0
62	7134.0
63	6264.0
64	5631.5
65	4999.0
66	3916.5
67	2834.0
68	2399.0
69	1964.0
70	1761.0
71	1558.0
72	1343.0
73	1128.0
74	888.5
75	483.5
76	318.0
77	243.0
78	168.0
79	130.0
80	92.0
81	75.0
82	58.0
83	34.5
84	11.0
85	9.0
86	7.0
87	3.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	289880.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.3533269893858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.01614285668107	26.543797167726336
2	10.435854177048801	7.5875603862360546
3	3.7567670073609643	4.097129383245884
4	2.08116597259045	3.0262922848305496
5	1.3460091053144694	2.4465954568093764
6	1.068717011187288	2.3310851380086333
7	0.8274434520729977	2.10562256649051
8	0.7127955672515461	2.072999226630415
9	0.6450049024704757	2.110326671633949
>10	6.013399067965433	38.6095878636986
>50	0.061808809933019675	1.5652584578166904
>100	0.02591982352029857	1.9537643774229632
>500	0.003987665156969011	0.9567683094781345
>1k	0.004984581446211264	4.593212709971922
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4569	1.5761694494273493	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTC	2315	0.7986063198564923	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCG	2278	0.7858424175520904	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGC	2134	0.7361666896646888	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	1378	0.47536911825583	No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	763	0.26321236373671864	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTTCGGAT	760	0.26217745273906445	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCT	560	0.19318338622878434	No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	557	0.1921484752311301	Illumina Single End Adapter 1 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	451	0.15558161998068168	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT	445	0.15351179798537326	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTC	412	0.14212777701117704	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTA	410	0.14143783634607424	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	403	0.13902304401821444	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC	343	0.1183248240651304	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.449703325514006E-4	0.0	0.0	0.19904788188215813	0.0
2	3.449703325514006E-4	0.0	0.0	1.1377121567545192	0.0
3	3.449703325514006E-4	0.0	0.0	1.4150683041258452	0.0
4	3.449703325514006E-4	0.0	0.0	1.885952808058507	0.0
5	3.449703325514006E-4	0.0	0.0	3.5321512349937905	0.0
6	3.449703325514006E-4	0.0	0.0	4.171381261211536	0.0
7	3.449703325514006E-4	0.0	0.0	4.943079895129019	0.0
8	3.449703325514006E-4	0.0	0.0	6.010763074375603	0.0
9	3.449703325514006E-4	0.0	0.0	6.372636953222023	0.0
10	3.449703325514006E-4	0.0	0.0	8.060231820063475	0.0
11	3.449703325514006E-4	0.0	0.0	9.405271146681386	0.0
12	3.449703325514006E-4	0.0	0.0	11.190147647302332	0.0
13	3.449703325514006E-4	0.0	0.0	11.650683041258452	0.0
14	3.449703325514006E-4	0.0	0.0	11.836967020836209	0.0
15	3.449703325514006E-4	0.0	0.0	12.30785152476887	0.0
16	3.449703325514006E-4	0.0	0.0	13.008831240513317	0.0
17	3.449703325514006E-4	0.0	0.0	13.816751759348696	0.0
18	3.449703325514006E-4	0.0	0.0	14.65778943010901	0.0
19	3.449703325514006E-4	0.0	0.0	15.462260245618877	0.0
20	3.449703325514006E-4	0.0	0.0	16.003173727059472	0.0
21	3.449703325514006E-4	0.0	0.0	16.58065406375052	0.0
22	3.449703325514006E-4	0.0	0.0	17.220574030633365	0.0
23	3.449703325514006E-4	0.0	0.0	17.83324134124465	0.0
24	3.449703325514006E-4	0.0	0.0	18.326203946460605	0.0
25	3.449703325514006E-4	0.0	0.0	18.763626328135782	0.0
26	3.449703325514006E-4	0.0	0.0	19.205188353801574	0.0
27	3.449703325514006E-4	0.0	0.0	19.648820201462673	0.0
28	3.449703325514006E-4	0.0	0.0	20.052090520215263	0.0
29	3.449703325514006E-4	0.0	0.0	20.491582723885745	0.0
30	3.449703325514006E-4	0.0	0.0	20.993514557748032	0.0
31	3.449703325514006E-4	0.0	0.0	21.4409410790672	0.0
32	3.449703325514006E-4	0.0	0.0	21.826962881192216	0.0
33	3.449703325514006E-4	0.0	0.0	22.242997102249205	0.0
34	3.449703325514006E-4	0.0	0.0	22.676279839933766	0.0
35	3.449703325514006E-4	0.0	0.0	23.1402649372154	0.0
36	3.449703325514006E-4	0.0	0.0	23.565613357251276	0.0
37	3.449703325514006E-4	0.0	0.0	23.991651717952255	0.0
38	3.449703325514006E-4	0.0	0.0	24.442527942596936	0.0
39	3.449703325514006E-4	0.0	0.0	24.941700013798812	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	20	7.0245506E-4	45.0	34
TACCACG	20	7.0245506E-4	45.0	1
TAGACGG	20	7.0245506E-4	45.0	2
GCGAACC	20	7.0245506E-4	45.0	33
CGCATGG	25	3.883565E-5	45.0	2
GGCGTAG	20	7.0245506E-4	45.0	1
TAACGTA	25	3.883565E-5	45.0	32
TCTAGCG	20	7.0245506E-4	45.0	1
ATTCCGC	20	7.0245506E-4	45.0	44
ACGATAG	20	7.0245506E-4	45.0	18
AGTGATT	35	1.207718E-7	45.0	42
CGTAGAA	20	7.0245506E-4	45.0	35
CTCATGT	25	3.883565E-5	45.0	15
GCGATTT	20	7.0245506E-4	45.0	9
ACGTAGA	20	7.0245506E-4	45.0	34
ATACGCA	20	7.0245506E-4	45.0	41
CGATCGA	45	3.8198777E-10	45.0	41
GCATGAC	20	7.0245506E-4	45.0	22
GTTGGTC	20	7.0245506E-4	45.0	11
GACCTTA	20	7.0245506E-4	45.0	9
>>END_MODULE