FastQCFastQC Report
Sat 18 Jun 2016
SRR3554440_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554440_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469514
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47631.0144532431407796No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC20830.4436502425912752No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGC20640.43960350490081235No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCG19080.40637765860016956No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC15970.34013895219311885No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC8590.18295514084777023No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT7180.15292408746065078No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGGTACAT6540.13929297102961785No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA5900.12566185459858492No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.10415024898086107No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCC4850.10329830420392151No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCA207.02902E-445.044
GCGCGAC253.8872684E-545.09
CAACCGG207.02902E-445.01
CGACCCG207.02902E-445.032
CGACAAT253.8872684E-545.020
AACGGCA207.02902E-445.020
GCCGATC207.02902E-445.043
GTATGCG253.8872684E-545.01
TACGAAT502.1827873E-1145.012
TCATCGA207.02902E-445.016
TTCGGAC253.8872684E-545.014
ACGTAAG351.2098826E-745.01
CGCACGG351.2098826E-745.02
CAATACA207.02902E-445.01
TTACGGG1150.043.043483
CGTTTTT25000.042.3900031
GTATTAG603.6379788E-1241.2499961
CGAATAT501.0786607E-940.514
CGTTATT2450.040.4081651
CCTCGTC451.924127E-840.036